技术概述
MALBAC®（Multiple Annealing and Looping Based Amplification Cycles）多次退火环状循环扩增技术，利用独特的具有链置换活性的DNA聚合酶进行准线性的全基因组预扩增，再通过PCR技术进行指数式扩增，为下游分析提供充足的实验材料。
MALBAC®（Multiple Annealing and Looping Based Amplification Cycles）多次退火环状循环扩增技术，利用独特的具有链置换活性的DNA聚合酶进行准线性的全基因组预扩增，再通过PCR技术进行指数式扩增，为下游分析提供充足的实验材料。
不同于以往的非线性或指数型扩增方法，MALBAC®技术利用特殊引物，使得扩增子的结尾互补而成环，从而很大程度上防止了DNA的指数性扩增。
Genome-wide detection of single-nucleotide and copy-number variations of a single human cell
Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing
Genome Analyses of Single Human Oocytes
Reproducible Copy Number Variation Patterns among Single Circulating Tumor Cells of Lung Cancer Patients
Validation of Multiple Annealing and Looping-based Amplification Cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos
Validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of blastocysts
Live Births after Simultaneous Avoidance of Monogenic Diseases and Chromosome Abnormality by Next-Generation Sequencing with Linkage Analyses
A New Next-generation Sequencing-based Assay for Concurrent Preimplantation Genetic Diagnosis of Charcot-Marie-Tooth Disease Type A1 and Aneuploidy Screening
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